The biosensor technology, which makes it possible to detect biomaterial such as protein, pathogen, and small molecules, is useful in such areas as diagnosis, bioprocessing, and food analysis or safety. For the development of a highly sensitive biosensor, immobilization techniques of organic/bio films on solid substrate, and detection methods of protein-protein reactions appearing in a few nanometers region from the sensor surface should be established. In this review, several immobilization techniques and detection methods are reviewed based on the articles reported recently.

• Young Min Bae(Institute of Applied Science and Technology, Sogang Unversity) Corresponding author
• Seong In Cho(School of Biological Resources and Materials Engineering, Seoul National University)
• Yong-Jin Cho(Biosystems Engineering Team, Korea Food Research Institute)