Transgenic lines of insect resistant cabbage (Brassica oleracea var. capitata) expressing Cry1Ac1 protein has been developed to control diamondback moth (Plutella xylostella). The potential adverse effects of Bt crops on non-target arthropod herbivores and predators are major concerns. We conducted a tritrophic bioassay to study the ecological impacts of insecticidal transgenic cabbage on the wolf spider (Pardosa astrigera), a non-target generalist predator. First, we measured the levels of Cry1Ac1 proteins in fruit flies that were fed with the transgenic cabbage as well as those levels in the wolf spiders preying on the Bt cabbage-fed fruit flies using enzyme linked immunosorbent assay (ELISA). Cry1Ac1 proteins were detected in the Bt cabbage fed fruit flies and also in the wolf spiders after preying on Bt cabbage-fed fruit flies. Second, we compared the life history characteristics of the wolf spiders preying on the Bt or non-Bt cabbage. Growth, development time and survival of the wolf spiders were not significantly different between Bt and non-Bt cabbage. Although the wolf spiders were exposed to Cry1Ac1 protein via feeding on the preys containing Cry1Ac1 proteins, their growth and survival was not significantly affected.

• Young-Joong Kim(Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Department of Agricultural Biotechnology, Seoul National University)
• Joon-Ho Lee(Department of Agricultural Biotechnology, Seoul National University)
• Chee Hark Harn(R&D Headquarter, Nongwoo Bio Co.)
• Chang-Gi Kim(Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)