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Developing New Microsatellite Markers of Sogatella furcifera by using NGS Technology

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/287695
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

White-backed planthopper, Sogatella furcifera (Horvath) (Hemiptera: Delphacidae), has been a serious migratory pest in Korea. It is important to figure out the migration route and gene flow of S. furcifera. Microsatellite marker (SSR) shows high efficiency as molecular markers. Unfortunately, various microsatellite marker of S. furcifera has not been developed to see genetic diversity. S. furcifera samples were collected from Vietnam, Laos and three different sites of Bangladesh in 2012. We extracted DNA by using QIAamp DNA Mini Kit and ran next generation DNA sequencer (NGS) Roche 454 to develop a new microsatellite marker. Roughly, about 18 singleton primers and 14 contigs primers were found. We will test these primers with S. fucifera DNA samples, and figure out the accurate new microstatellite marker.

저자
  • Hwa Yeun Nam(Department of Agricultural Biotechnology, Seoul National University)
  • Brad Coates(USDA–ARS, CICGRU, Genetics Laboratory, Iowa State University)
  • Kyung Seok Kim(USDA–ARS, CICGRU, Genetics Laboratory, Iowa State University)
  • Chris Wright(W.M. Keck Center, University of Illinois at Urbana-Champaign)
  • Marana Park(Department of Agricultural Biotechnology, Seoul National University)
  • Joon-Ho Lee(Department of Agricultural Biotechnology, Seoul National University)