To investigate the trophic relationship between the fresh water fishes and aquatic invertebrates, the research of their prey is important. However, the morphological identification of the prey in the fish intestine is very difficult due to the process of digestion. Thus, we conducted the DNA barcoding methods for aquatic insect samples from the gut of fresh water fishes for identifying hardly recognizable species.
All benthic macroinvetebrates and manchurian trouts were collected from the upper streams of Inje-gun, Korea, in spring and summer, 2013. A total of 27 unique taxa were recognized, among which 23 species were identified by morphology, and 4 unrecognizable individuals were identified based on mitochondrial cytochrome C oxidase I (Mt-COI). As the results of this study, Ephemerella sp., Neophylax sp., Neophylax sp., and Pteronarcys sp. are verified to the genus level by DNA barcoding.

• Geonho Cho(School of Agricultural Biotechnology, Seoul National University)
• Seunghwan Lee(School of Agricultural Biotechnology, Seoul National University)