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Identification of the Diadegma fenestrale Ichnovirus (DfIV) and Analysis Results of Genome Draft

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  • URLhttps://db.koreascholar.com/Article/Detail/288859
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The Diadegma fenestrale was known as parasitoid on potato tuber moth, Phthorimaea operculella and diamondback moth, Plutella xylostella. This species, genus Diadegma are first reported from Korea. DfIV showed typical ichnovirus shape which two membranes surround virus capsids. The genome contents of DfIV consist about sixteen double-stranded DNA segments ranging 2 to 6 kb. To identify DfIV genes, whole genome sequencing based on GS-FLX was conducted using purified total viral DNA extracted from D. fenestrale calyx. About sixty ORFs were analyzed and several typical polydna virus gene family detected such as cys-motif, rep, vinnexin and vankyrin. This is the first report of DfIV and these lepidopteran host immune suppression genes will be deeply identified.

저자
  • Ju Il Kim(Highland Agriculture Research Center, NICS, RDA)
  • Min Kwon(Highland Agriculture Research Center, NICS, RDA)
  • Yong Gyun Kim(Department of Bioresource Sciences, Andong National University)
  • Si Hyeock Lee(Department of Agricultural Biotechnology, Seoul National University)
  • Jin Kyung Choi(National Institute of Environmental Research)
  • Jong Wook Lee(Department of Life Sciences, Yeungnam National University)