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A Case Study: Why do not Match Morphological Data and DNA Barcoding in the Korean Hatchiana Fender, 1966 (Coleoptera: Cantharidae)

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  • URLhttps://db.koreascholar.com/Article/Detail/290640
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

DNA barcode is known to be successfully applied in identification on the members of Insecta. In recent studies, however, it was known that the DNA approach may fail in several taxa with following cases: (1) very recent speciation and hybridization, (2) recent diverged groups with complex gene histories, (3) the spread of maternally transmitted bacteria, (4) adding more than one geographical race and at least one congener, (5) different levels of dispersal. In this study, we taxonomically review on the Korean Hatchiana using the morphological data and DNA barcodes. In morphology, they are distinct from each other by the characteristics of body coloration, eye, pronotum, scutellum, and aedeagus. In molecular data, however, the interspecific sequence distance ranged from 0.0-3.4%. This result is caused by H. glochidiatus, of which the sequence divergence is 0.2-2.8% in H. rosinae, 0.8-2.6% in H. baekripoensis, and 0.0-3.3% in H. jirisanensis. Also, H. glochidiatus produces mixed-clusters with H. rosinae and H. jirisanensis in NJ phenogram. Through this presentation, therefore, we discuss on why the four Korean Hatchiana species distinct by morphological characters produce mixed-clusters in DNA barcoding.

저자
  • Kang, Tae Hwa(College of Agriculture & Life Sciences, Chonnam National University)
  • Jin Ill Kim(Applied Entomology Division, Department of Agricultural Biology, NAAS)
  • Mi Ae Kim(College of Agriculture & Life Sciences, Chonnam National University)
  • Iksoo Kim(Dept. of Biology, Sungshin Women's University)
  • Hae Chul Park(College of Agriculture & Life Sciences, Chonnam National University)