Preharvest sprouting (PHS) not only causes reduction of grain yield, but also affects the quality of grains, resulting into significant economic losses. PHS is governed by multiple genes. Little is known about the large genetic variation of preharvest sprouting in rice. In the present study, genetic variations of four PHS genes, OsVP1, Osaba1, Alpha-amylase3D and OsGA20ox1 were studied by using whole-genome resequencing data of 84 accessions of rice core set. A total of haplotype groups; 27, 29, 6 and 14, for OsVP1, Osaba1, Alpha-amylase3D and OsGA20ox1, respectively, were detected among the 84 accessions. Some new SNPs and InDels were found in exon part of PHS related genes were expected to result in amino acid changes following analysis of the genetic code variations, and the germplasm or varieties which are resistant to preharvest sprouting were explored. Based on this step, phenotyping for PHS is ongoing, and the association mapping of PHS will be conducted by using SNPs resulted from the haplotyping data. The present results will be ultimately useful to the molecular breeding for the development of PHS resistant rice cultivars.

• Yong-Jin Park(Department of Plant Resources, College of Industrial Science, Kongju National University, Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University) Corresponding Author
• Aye Aye Khaing(Department of Plant Resources, College of Industrial Science, Kongju National University)