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Expression profiling and genome mining for selected candidate genes for heat and cold tolerance in ri

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한국육종학회 (The Korean Breeding Society)
초록

To obtain a better insight into a possible common regulatory mechanism shared in response to heat and cold stresses in model monocot plant, expression profiling for several selected transcripts of rice (O. sativa L.) was examined from meiotic stage panicle samples of a number of different cultivars including both heat/cold tolerant and susceptible varieties. Induction of several of these genes in response to heat stress treatment was observed across all different rice varieties tested, but no differential induction kinetics between the heat-tolerant and susceptible varieties was discernable for these genes. On the other hand, a few genes that are known to be involved in ROS signaling showed different induction kinetics between the tolerant and susceptible varieties, suggesting that these genes might be playing a key role in conferring the heat/cold stress tolerance at reproductive stage of tolerant rice varieties. This approach was also augmented by comparative gene expression data analyses, utilizing expression data of other monocots and dicots model plants available in the database. Based upon these results, a preliminary gene pyramiding effort to estimate contributing factor of several selected alleles after putting these genes together is underway. This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No.PJ009076), Rural Development Administration, Republic of Korea.

저자
  • Sunghan Kim(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Dong-Ryung Lee(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Hee-Jong Koh(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University) Corresponding Author