Bt gene derived from the B. thuringiensis has been used for developing GM crops, and corn, cotton and soybean producing B. thuringiensis toxins have been on the market for last 16 years or so creating a huge GMO industry. One of the notorious pests in brassica crops is diamond backmoth (DBM). In order to protect the insect plague of crops from DBM, 4-5 billion dollars have been wasted annually for applying integrated measures in worldwide. Major prevention is use of pesticides that may build the contamination level of chemicals in the ground and this practice threats the environment and ecosystem. An alternative is to develop GM brassica crops and therefore we have developed GM cabbages resistant DBM using bt gene. Lots of T0 cabbages were tested for resistance and independent GM cabbages resistant to DBM were selected. Molecular analysis was conducted to find GM cabbage to hold one copy transgene and intergenic insertion. We found two independent GM cabbages as an event and those have been self-crossed for two generation. Also we are working the development of GM cabbage with different vector that contains bar gene as a selection marker.

• Bo-Min Kim(Biotechnology Institute, Nongwoo Bio Co.)
• Jong-Sup Shin(Breeding Institute, Nongwoo Bio Co.)
• Beom-Seok Park(Brassica Genomics Team, NIAB of RDA)
• Soon-Chun Jeong(Bio-Evaluation Center, KRIBB)
• Chee-Hark Harn(Biotechnology Institute, Nongwoo Bio Co.) Corresponding Author