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Isolation of Pepper Genes Interacting with CMV Helicase Domain via Yeast Two Hybrid Screening
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/298025
Cucumber mosaic virus (CMV), which has the broadest host range among plant viruses, is a very destructive pathogen in pepper production. Various resistance sources against CMV have been identified by plant breeders. One of them is Capsicum annuum ‘Bukang’, which contains a single dominant resistance gene, Cmr1. C. annuum ‘Bukang’ is resistant to CMV-P0 strains (CMV-Kor and CMV-Fny), but susceptible to CMV-P1. CMV-P1 is a new strain recently identified in South Korea. Previously we showed that CMV-P1 RNA1 helicase domain is responsible for overcoming Cmr1 and may be play a role in viral replication and systemic infection. To identify the plant host factors involved in CMV-P1 replication and movement, we utilized a yeast two-hybrid system derived from C. annuum ‘Bukang’ cDNA library as a prey. A total of 78 potential host genes interacting with the CMV-P1 RNA1 helicase domain were isolated in the first screening, and PCR confirmation and sequencing analysis narrowed the candidates to ten genes. The candidate genes have found to be encoding acireductone dioxygenase, ADP-glucose pyrophosphorylase, ADP-ribosylation factor 1, ADP-ribosylation factor, calreticulin-3 precursor, cysteine synthase, formate dehydrogenase, histone-H3, phosphomannomutase and polyubiqutin 6PU11. Previous studies showed that these genes were involved in virus infection, replication or virus movement. To elucidate the function of these genes, VIGS and coimmumo precipitation assay is being done.
