Miraculin can modify a sour taste into a sweet taste and is an alternative to traditional sweeteners. Mirculin gene was introduced into the Miyagawa Wase Satsuma mandarin (Citrus unshiu Marc.) callus by Agrobacterum-mediated transformation to produce citrus transgenic plant. Transgenic plant candidates were selected via hygromycin resistance. Transformation was confirmed by Polymerase Chain Reaction, Southern blotting, and Western blot analysis. Expression of this gene in transgenic citrus resulted in the accumulation of miraculin protein in the leaves. Multiple Shoots of transgenic citrus planets were micrografted onto trifoliate rootstocks in the sterile soil. Plants were established in the greenhouse 2 years after planting.

• Fei Li(Faculty of Biotechnology, Jeju National University)
• Seong Beom Jin(Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
• Myeung-Seung Kim(Subtropical Horticulture Research Institute, Jeju National University)
• Song-I Han(Subtropical Horticulture Research Institute, Jeju National University)
• Hyeon-Jin Sun(Subtropical Horticulture Research Institute, Jeju National University)
• Jae Hoon Kim(Faculty of Biotechnology, Jeju National University, Subtropical Horticulture Research Institute, Jeju National University) Corresponding Author