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Soybean germplasm, a rich genetic resource to be explored for the identification of salt tolerance genes and their mechanism of action

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한국육종학회 (The Korean Breeding Society)
초록

Soybean germplasm have diverse accessions with great variation in their ability to survive and reproduce under salt stress conditions. In general, cultivated soybeans are more sensitive to salt stress than their wild relatives, however exceptions are found in both the groups. These variations in response to salt stress makes soybean germplasm an interesting collection of genetic resources to be explored for the identification of salt-tolerance genes, and their mechanism of action. Here, in this report we presented a data showing differential response of selected accessions of both cultivated and wild soybeans to salt stress. Two modes of salt treatment; gradual salt stress (GS) as well as salt shock (SS) were used in this study. The GS was found more effective in finding the difference in response of soybean accessions to salt stress. Various genetic marker based methods are in use to identify and isolate the potential genes contributing to the salt tolerance in soybean. Even then there is a paucity of knowledge on the key genes contributing to the salt tolerance in soybean. We expect that a recently developed functional screen based method, like yeast based functional screen, using cDNA library generated from different salt tolerant accessions of soybean could lead to identification of novel genes responsible for salt tolerance in soybean. Also, we propose for the use of RNA isolated from different stages of GS and SS for making cDNA library to be used for functional screening.

저자
  • Sajeesh Kappachery(School of Applied Biosciences, Kyungpook National University)
  • Jagadeesh Sundaramoorthy(School of Applied Biosciences, Kyungpook National University)
  • Gyu Tae Park(School of Applied Biosciences, Kyungpook National University)
  • Jeong-Dong Lee(School of Applied Biosciences, Kyungpook National University)
  • Hak Soo Seo(Department of Plant Bioscience, Seoul National University, Bio-MAX Institute, Seoul National University)
  • Jong Tae Song(School of Applied Biosciences, Kyungpook National University) Corresponding Author