The aim of the present study was to identify coat color associated genes that are differentially expressed in mature Korean brindle cattle (KBC) with different coat colors and in Hanwoo cows. KBC calves, before and after coat color appearance, were included. Total cellular RNA was isolated from the tail hair cells and used for microarray. The number of expressed coat color associated genes/probes was 5813 in mature KBC and Hanwoo cows. Among the expressed coat color associated genes/probes, 167 genes were the coat color associated genes listed in the Gene card database and 125 genes were the pigment and melanocyte genes listed in the Gene ontology_bovine database. There were 23 genes/probes commonly listed in both databases and their expressions were further studied. Out of the 23 genes/probes, MLPH, PMEL, TYR and TYRP1 genes were expressed at least two fold higher (p<0.01) levels in KBC with brindle color than either Hanwoo or KBC with brown color. TYRP1 expression was 22.96 or 19.89 fold higher (p<0.01) in KBC with brindle color than either Hanwoo or KBC with brown color, respectively, which was the biggest fold difference. The hierarchical clustering analysis indicated that MLPH, PMEL, TYR and TYRP1 were the highly expressed genes in mature cattle. There were only a few genes differentially expressed after coat color appearance in KBC calves. Studies on the regulation and mechanism of gene expression of highly expressed genes would be next steps to better understand coat color determination and to improve brindle coat color appearance in KBC.

INTRODUCTION
 MATERIALS AND METHODS
  1. Experimental Animals and RNA Extraction
  2. Microarray Analys
 RESULTS
  1. Gene Expression in Korean Brindle Cattle with Different Coat Colorsand in Hanwoo
  2. Gene Expression in Korean Brindle Cattle Calves before and afterCoat Color Expression
 DISCUSSION
 REFERENCES

• Hae-Lee Lee(Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research, Jinan-gun 567-891, Korea)
• Jae-Hee Park(Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research, Jinan-gun 567-891, Korea, Department of Animal Sciences, College of Agriculture and Life Science, Institute of Molecular Biology and Genetics,Chonbuk National University, Jeonju 561-756, Korea)
• Jong Gug Kim(Department of Animal Sciences, College of Agriculture and Life Science, Institute of Molecular Biology and Genetics,Chonbuk National University, Jeonju 561-756, Korea) Correspondence