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기내배양을 통한 황기 부정근의 생산과 유효성분 분석 KCI 등재

Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus

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  • URLhttps://db.koreascholar.com/Article/Detail/309296
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한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones.
Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with 1.0㎎• ℓ −1 indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O-β-D-glucopyranoside was higher than that of other adventitious root samples.
Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.

목차
서 언
재료 및 방법
  1. 식물재료
  2. 부정근 생산을 위한 황기 캘러스 유도
  3. 분석 시약 및 기기
  4. 추출조건
  5. 분석조건
  6. 통계분석
결과 및 고찰
  1. 황기 기내배양을 위한 부정근 유도
  2. 유효성분 분석
감사의 글
REFERENCES
저자
  • 허 목(농촌진흥청 국립원예특작과학원 인삼특작부) | Mok Hur
  • 이대영(농촌진흥청 국립원예특작과학원 인삼특작부) | Dae Young Lee
  • 이재원(농촌진흥청 국립원예특작과학원 인삼특작부) | Jae Won Lee
  • 안태진(농촌진흥청 국립원예특작과학원 인삼특작부) | Tae Jin An
  • 이정훈(농촌진흥청 국립원예특작과학원 인삼특작부) | Jeong Hoon Lee
  • 김영국(농촌진흥청 국립원예특작과학원 인삼특작부) | Young Guk Kim
  • 차선우(농촌진흥청 국립원예특작과학원 인삼특작부) | Seon Woo Cha
  • 엄유리(농촌진흥청 국립원예특작과학원 인삼특작부) | Yurry Um Corresponding author