The hazards associated with the polycyclic aromatic hydrocarbons (PAHs) are known to be recalcitrant by their structure, but white rot fungi are capable of degrading recalcitrant organic compounds. Phlebia brevispora KUC9045 isolated from Korea was investigated its efficiency of degradation of four PAHs, such as phenanthrene, anthracne, fluoranthene, and pyrene. And the species secreted extracellular laccase and MnP (Manganese dependent peroxidase) during degradation. P. brevispora KUC9045 demonstrated effective degradation rates of phenanthrene (66.3%), anthracene (67.4%), fluoranthene (61.6%), and pyrene (63.3%), respectively. For enhancement of degradation rates of PAHs by the species, Remazol Brilliant Blue R (RBBR) was preferentially supplemented to induce ligninolytic enzymes. The biodegradation rates of the three PAHs including phenanthrene, fluoranthene, and pyrene were improved as higher concentration of Remazol Brilliant Blue R was supplemented. However, anthracene was degraded with the highest rate among four PAHs after two weeks of the incubation without RBBR addition. According to the previous study, RBBR can be clearly decolorized by P. brevispora KUC9045. Hence, the present study demonstrates simultaneous degradation of dye and PAHs by the white rot fungus. And it is considered that the ligninolytic enzymes are closely related with the degradation. In addition, it indicated that dye waste water might be used to induce ligninolytic enzymes for effective degradation of PAHs.

Introduction
 Materials and Methods
  1. Chemicals
  2. Organisms and medium
  3. Experimental design
  4. Analytical methods
  5. Enzyme assays
  6. Data analysis
 Results and Discussion
  1. Comparison of P. brevispora with controls onPAHs degradation
  2. T ime course of PAHs degradation byP. brevispora
  3. Ligninolytic enzyme during degradation of PAHs
  4. E nhanced degradation of PAHs withdecolorization of RBBR
 Conclusion
 References

• Aslan Hwanhwi Lee(Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea)
• Hanbyul Lee(Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea)
• Jae-Jin Kim(Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea) Corresponding author