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두록 정자의 운동학적 특성과 후보 유전자 ESR2 유전적 다형성과의 연관성 분석 KCI 등재

Investigation on Association of ESR2 polymorphism as a Candidate Gene for Duroc sperm motility and kinematic characteristics

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/317353
  • DOIhttps://doi.org/10.12750/JET.2016.31.3.287
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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Estrogen receptors 2(ESR2) is involved in estrogen related apoptosis in cell cycle spermatogenesis, but their functions have not been confirmed in pig until now. Therefore, this study was conducted to analyze their association with sperm motility and kinematic characteristics. DNA samples from 105 Duroc pigs with records of semen motility and kinematic characteristics [Total motile spermatozoa (MOT), Curvilinear velocity(VCL), Straight-line velocity(VSL), the ratio between VSL and VCL(LIN), Amplitude of Lateral Head displacement(ALH)] were analyzed. A SNP in coding region of ESR2 g.35547A > G in exon 5 was associated with MOT (p < 0.05) in Duroc population. Therefore, we suggest that the porcine ESR2 gene may be used as a molecular marker for Duroc boar semen quality, although its functional effects were not defined yet. These results might shed new light on the roles of ESR2 in spermatogenesis as candidate gene for boar fertility, but still the lack of association across populations should be considered.

목차
서 론
 재료 및 방법
  정액 채취
  정자 운동학적 특성 분석
  Direct sequencing 및 유전자형 분석
  통계 분석
 결과 및 고찰
 REFERENCES
저자
  • 정용대(농촌진흥청 국립축산과학원) | Yong-dae Jeong
  • 정진영(농촌진흥청 국립축산과학원) | Jin-Young Jeong
  • 사수진(농촌진흥청 국립축산과학원) | Soo-Jin Sa
  • 김기현(농촌진흥청 국립축산과학원) | Ki-Hyun Kim
  • 조은석(농촌진흥청 국립축산과학원) | Eun-Seok Cho
  • 유동조(농촌진흥청 국립축산과학원) | Dong-Jo Yu
  • 최정우(단국대학교 약학대학) | Jung-Woo Choi
  • 장현준(단국대학교 약학대학) | Hyun-Jun Jang
  • 우제석(농촌진흥청 국립축산과학원) | Jae-Seok Woo Correspondence
  • 박성권(세종대학교 생명과학대학 식품생명공학과) | Sungk-won Park Correspondence