Dengue is the most important arboviral human infection worldwide. Approximately 50-100 million cases occur annually and more than 2.5 billion people are at risk of infection. This virus is possibility of overseas inflow to be high in Korea. We collected mosquitoes in Jeju, Busan, Gunsan, and Incheon using BG Sentinel trap from April to August in 2016. Collected mosquitoes were conducted virus detection using real-time PCR method. A total of 15 species comprising 7 genera were identified and 3,696 female mosquitoes collected. The most dominant species ratio (SR) was 49.4% (Culex pippins complex) followed by 21.8% (Aedes albopictus), 13.6% (Ae. vexans) and Ochlerotatus dorsalis (4.8%). Dengue virus was not detected in any mosquitoes until now.