In the present study, we examined potential roles of glucose and pyruvate in nuclear and cytoplasmic maturation of porcine oocytes. In the presence and absence of 10% porcine follicular fluid (PFF), either 5.6 mM glucose or 2mM pyruvate effect on meiotic maturation and followed development ability. However, DOs doesn't take full advantage of the glucose in medium, only pyruvate can increase MII rate and follow early embryo development ability significance. COCs were matured with 200 uM pentose phosphate pathway (PPP) inhibitor (dehydroepiandrosterone, DHEA) or 2 μM glycolysis inhibitor (iodoacetate, IA), significantly lower levels of GHS in the DHEA an IA treated oocytes and the levels of ROS were higher significantly in the DHEA treated oocytes, treatment with DHEA significantly reduced the intra-oocyte ATP and NADPH level. Blastocysts from DHEA or IA treated group also presented higher apoptosis levels, meanwhile, the percentage of proliferating cells was dramatically lower than the non-treated group. In conclusion, our results suggest that 10% PFF promoted oocytes make full use of energy, glucose metabolism during in vitro maturation inseparable from the cumulus cells, PPP and glycolysis promoted porcine oocytes cytoplasmic maturation by supplying energy and reducing oxidative stress.

• Bao Yuan(Department of Laboratory Animal Science, College of Animal Sciences, Jilin University, Changchun 130062, China, Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)
• Shuang Liang(Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)
• Jiabao Zhang(Department of Laboratory Animal Science, College of Animal Sciences, Jilin University, Changchun 130062, China)
• Jeong-Woo Kwon(Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)
• Shun-Ha Park(Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)
• Hai-Yang Wang(Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)
• PIAO XUAN JING(Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)
• Xiang-Shun Cui(Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)
• Nam-Hyung Kim(Molecular Embryology Laboratory, Department of Animal Sciences, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University, South Korea)