Most traits of crop are quantitative, which are controlled by polygenes. Finely mapping ofquantitative trait loci (QTLs) is very important for cotton genetic improvement. Chromosome segment substitution lines (CSSLs) containing only one or a few segments of donor chromosome introgressed into a recipient genetic background can be used for QTL fine mapping and map-based cloning. In this study, the Gossypium. hirsutum variety ‘ZM36’ and Gossypium. barbadense ‘Hai1’ were respectively used as recurrent and donor parent to produce a segment substitution lines populations, including the BC4F2:3 and BC4S2:4 generations, by using microsatellite marker-assisted selection (MAS). We obtained 283 SSR marks by taking about the average 5 cM distance selection marks on this map, and these marks were carried to the molecular examination with to two populations. The results showedthat recurrent parent genetic background already to restore the very high degree through the high generation of backcross.

• Changbo Dai(Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
• Yujuan Jia(Henan Institute of Science and Technology)
• Qing-Lian Wang(Henan Institute of Science and Technology)
• Myeong-Hyeon Wang(Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) Corresponding author