Samples were taken from the surface waters around Dokdo Island to measure the physico-chemical and microbiological water qualities from June 23 to 26, 2007. Water temperature and dissolved oxygen were in the range of 20.7~20.9oC and 7.01~7.74, respectively. Salinity and pH were in the range of 34.5~34.8‰ and 8.23~8.37, respectively. Population densities of aerobic oligotrophic bacteria and heterotrophic bacteria by plate counting method were in the range of 2.4±0.5×103~3.0±0.3×103 and 4.4±0.6×103~6.0±0.9×103 cfu ml−1, respectively. Among thirty-seven isolates isolated from surface waters around Dokdo island by using 16S rDNA analysis, dominant species were closely related to Actionobacteria (7 clones), α-proteobacteria group (6 clones), and γ-proteobacteria group (4 clones).