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캐나다 옥수수 자식계통들에 대한 유전적 다양성 및 집단구조 분석 KCI 등재

Analysis of Population Structure and Genetic Diversity among Canadian Maize Inbred Lines using SSR Markers

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한국육종학회지 (Korea Journal of Breeding Science)
한국육종학회 (The Korean Breeding Society)
초록

We collected 32 maize inbred lines from eastern cereal and oilseed research center in Canada to develop new maize varieties. We also evaluated genetic diversity, genetic relationships, and population structure using 35 SSR markers. A total of 269 alleles were revealed in 35 loci with an average of 7.69 and a range between 3 and 15 alleles per locus. The genetic diversity values varied from 0.176 to 0.889 with an average of 0.691. The polymorphic information content varied from 0.171 to 0.879 with an average of 0.659. Population structure analysis indicated that 32 Canadian maize inbred lines comprised four major groups and one admixed group based on a membership probability threshold of 0.80. The four major groups contained 13, 2, 5 and 2 maize inbred lines, respectively. From genetic relationships analysis, the all inbred lines were divided into three main groups at 26% genetic similarity. Group I included 22 inbred lines, and Group II included 9 inbred lines. Group III consist of only one inbred line. The results in this study would be useful for the improvement and development of new cultivars, planning crosses for hybrids or development of inbred line in maize breeding program

목차
서 언
 재료 및 방법
  식물 재료 및 DNA 추출
  SSR 분석 및 PCR 증폭
  전기영동 및 silver-staining
  Data 통계분석
 결과 및 고찰
  캐나다 옥수수 자식계통들의 SSR 변이성
  캐나다 옥수수 자식계통들의 집단구조와 계통유연관계
 적 요
 사 사
 REFERENCES
저자
  • 홍탁기(강원대학교 농업생명과학대학 식물자원응용공학과) | Tak Ki Hong
  • 사규진(강원대학교 농업생명과학대학 식물자원응용공학과) | Kyu Jin Sa
  • 이주경(강원대학교 농업생명과학대학 식물자원응용공학과) | Ju Kyong Lee Corresponding Author