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가시엉겅퀴의 부위별 항산화 활성과 화학적 구성성분 비교
A Comparative Study on Antioxidant Activity and Chemical Composition of Different Parts of Cirsium japonicum
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/326646
Background : Cellular damage caused by excessive reactive oxygen species (ROS) and peroxynitrite (ONOO-) generations has been implicated in several human diseases. The present study was carried out to evaluate the in vitro ROS and ONOO- scavenging activities of Cirsium japonicum parts. Methods and Results : The dried Cirsium japonicum parts (whole plant, leaf, seed coat) were extracted by EtOH, at room temperature. In order to determine antioxidant activity of Cirsium japonicum parts, we were carried out ROS and ONOO- scavenging analyses. As a result , the IC50 in ROS scavenge were showed 203.99 ± 22.04 μg/ml, 174.44 ± 7.78 μg/ml, 86.77 ± 7.02 μg/ml. The IC50 in ONOO- scavenge were showed 15.68 ± 0.57 μg/ml, 12.99 ± 0.15 μ g/ml, 10.33 ± 0.19 μg/ml, respectively. Active compound content in C. japonicum was determined using a HPLC/UV, reverse-phase column with gradient elution program (water in 0.5% formic acid : acetonitrile = 100:0 to 0:100 for 45 min, 0.8 ml/min). UV detection was conducted at 340 nm. The content of apigenin was measured in whole plant (1.04±0.06 mg/g), leaf (0.91±0.02 mg/g), seed coat (33.33±0.93 mg/g). Conclusion : Each part of Cirsium japonicum was analyzed antioxidant activity and the content of apigenin with EtOH extract. In result antioxidant activity, seed coat > leaf > whole plant. Seed coat were showed a very strong antioxidant activity. The comparative patterns between the antioxidant capacity and HPLC analysis results for the apigenin contained in Cirsium japonicum may also prove to be significant.
