논문 상세보기

섬유소분해효소에 의한 뽕잎 추출물내 항당뇨성분의 생물전환

Bio-Conversion of Hypoglycemic Constituents in the Leaves Extracts of Morus alba L by Celluolytic Enzymes

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/326673
서비스가 종료되어 열람이 제한될 수 있습니다.
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : The hypoglycemic effects of mulberry leaves extract were evaluated by comparing the abilities on glucose uptake in C2C12 myotubes and 3T3-L1 adipocyte. Glucose uptake of the extracts were identified to be enhanced by bio-conversion using cellulolytic enzyme like Viscozyme. Methods and Results : The mulberry (Morus alba L) leaves were extracted with 30% ethanol or hot water. The hypoglycemic compounds such as Moracin C and, Quercetin and 1-Deoxynojirimycin were identified from the extracts of mulberry leaves. The extracts were fermented using kinds of celluolytic enzymes, which were vicozyme, pectinase, β-glucosidase and xylanase, in order to increase the contents of hypoglycemic constituents in the extracts. The hypoglycemic effects of the fermented extracts were evaluated by comparing the abilities on glucose uptake in C2C12 myotubes and 3T3-L1 adipocyte. The extracts of mulberry leaves fermented with only Viscozyme were identified to increase glucose uptake in C2C12 myotubes and 3T3-L1 adipocyte by supplement of the concentration of 10 μM extracts, compared to insulin as control. However, bio-conversion effects by other enzymes were not shown in the treatments, suggesting hypoglycemic constituents in the extracts of mulberry leaves can be conversed to more active compounds by cellulolytic enzyme treatment like viscozyme. Conclusion : From the above results, we may suggest that the hypoglycemic constituents in mulberry leaves extracts can be conversed to more active compounds by cellulolytic enzymes.

저자
  • Chang Hoon Han(Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences) | 한창훈
  • Heon Hak Lee(Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences) | 이헌학
  • Jong Seong Kang(Department of pharmacy, College of pharmacy, Chungnam National University) | 강종성
  • Young Ho Kim(Department of pharmacy, College of pharmacy, Chungnam National University) | 김영호
  • Chang Seon Myung(Department of pharmacy, College of pharmacy, Chungnam National University) | 명창선
  • Min Ho Yoon(Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences) | 윤민호 Corresponding author