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개병풍 추출물의 항산화 및 항염 활성 검정

Anti-Oxidant and Anti-Inflammatory Activities of Astilboides tabularis (Hemsl.) Engl.

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  • URLhttps://db.koreascholar.com/Article/Detail/326686
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : Astilboides tabularis (Hemsl.) Engl. is a perennial herbaceous plant, distributed in the northern high mountains of the Korean peninsula and China. It is an excellent ornamental plant currently at risk of overharvesting and therefore, is designated as an endangered wild plant Class II by the Ministry of Environment. Physiological research on A. tabularis has not be reported. Therefore, in this study, using A. tabulari extracts, antioxidant and Anti-inflammatory effects were determined. Methods and Results : The antioxidant and free radical scavenging activities of A. tabularis extracts were evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The results showed that the ethyl acetate fraction of A. tabularis possesses potent DPPH radical scavenging activity (2.90±0.08㎍/㎖), similar to the scavenging activity of ascorbic acid (2.19±0.06㎍/㎖), and better than the powerful antioxidant α-tocopherol (10.60±0.40㎍/㎖) as well as BHA (butylatedhydroxy anisole)(6.12±0.27㎍/㎖). The ethyl acetate fraction possessed a significantly higher concentration of total phenolic (549.70±2.72㎎GAE/g) and flavonolic content (154.58±1.04㎎QE/g). It was also found that the ethyl acetate fraction exhibited high reducing power and inhibition of ROS (Reactive Oxygen Species) formation. Different fractions of A. tabularis were tested for anti-inflammatory activity using LPS stimulated Raw 264.7 cells. The n-hexane and ethyl acetate fractions exhibited a high inhibitory effect on NO (Nitrite oxide) production (22.43±1.06%, 19.30±0.45%, respectively) at 200㎍/㎖ concentration. The mRNA of IL-1β, iNOS and COX-2 gene expression was decreased by treatment with the ethyl acetate fraction. These results showed that A. tabularis extracts can be used as natural substances to control inflammation. Conclusion : These result showed that A. tabularis extracts can be used in a variety of antioxidant and other functional product research and development processes as valuable natural materials.

저자
  • Chan Ok Lee(Department of Applied Plant Sciences, Kangwon National University) | 이찬옥
  • Hee Kyu Kim(Gangwon Nature Environment Research Park) | 김희규
  • Ju Hee Park(Department of Applied Plant Sciences, Kangwon National University) | 박주희
  • Eun Yeong Choi(Department of Applied Plant Sciences, Kangwon National University) | 최은영
  • Yong Soo Kwon(Department of Pharmacy, Kangwon National University) | 권용수
  • Chang Yeon Yu(Department of Applied Plant Sciences, Kangwon National University) | 유창연
  • Myong Jo Kim(Department of Applied Plant Sciences, Kangwon National University) | 김명조 Corresponding author