Content analysis of loliolide in the leaves of Boehmeria nivea (Bn) collected from different region during four months (June, July, August, and September) was conducted by HPLC. The content of loliolide was detected in the leaves of B. nivea from Bns-2, -7, -10, -23, -38, -41, -67, -76, and -90 in June (5.02, 6.35, 6.93, 5.89, 4.31, 4.24, 4.91, 5.12, and 5.46 mg/g, respectively), July (4.32, 6.42, 7.72, 7.97, 4.05, 4.32, 5.65, 6.67, and 5.39 mg/g, respectively), August (3.52, 5.17, 3.90, 4.27, 3.26, 4.72, 3.82, 3.30, and 3.31 mg/g, respectively), and September (7.04, 7.25, 7.43, 7.86, 6.76, 6.38, 7.60, 6.79, and 4.77 mg/g, respectively). Among them, the highest content of loliolide was found in Bn-23 and in September. These results may be useful in determining the optimal harvest time at which phytochemical reaches a maximum level.

• Sunghun Cho(Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea)
• Jaemin Lee(Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea)
• Sanghoon Yang(Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea)
• Yong-Su Jung
• Ho Bang Kim(Life Sciences Research Institute, Biomedic Co. Ltd., Bucheon 420-852, Korea)
• Eun Ju Cho(Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Korea)
• Sanghyun Lee(Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea)