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기내 배양을 통한 국내 콩(Glycine max L.) 품종의 식물체 재분화 KCI 등재

Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)

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한국자원식물학회지 (Korean journal of plant resources)
한국자원식물학회 (The Plant Resources Society Of Korea)
초록

Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.

저자
  • 김동건(순천대학교 대학원) | Dong-Gun Kim (Graduate School, Sunchon National University)
  • 비파다 칸타요스(순천대학교 대학원) | Vipada Kantayos (Graduate School, Sunchon National University)
  • 김동관(전남 농업기술원 식량작물연구소) | Dong-Kwan Kim (Institute of Agricultural Science and Technology, Jellaname-do Agricultural Research and Extension Services)
  • 박흥규(전남 농업기술원 식량작물연구소) | Heung-Gyu Park (Institute of Agricultural Science and Technology, Jellaname-do Agricultural Research and Extension Services)
  • 김행훈(순천대학교 웰빙자원학과) | Haeng-Hoon Kim (Department of Well-being Bioresources, Sunchon National University)
  • 나의식(순천대학교 웰빙자원학과) | Eui-Shik Rha (Department of Well-being Bioresources, Sunchon National University)
  • 이성춘(순천대학교 웰빙자원학과) | Sheong Chun Lee (Department of Well-being Bioresources, Sunchon National University)
  • 배창휴(순천대학교 웰빙자원학과) | Chang-Hyu Bae (Department of Well-being Bioresources, Sunchon National University) Corresponding author