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An investigation of the effects of Pb for domestic anuran embryos were evaluated with the Frog Embryo Teratogenesis Assay; Xenopus (FETAX). Depending on the species, the difference between the embryo size and the embryonic development time was determined. As a result, mortality and malformation rates were increased, malformation patterns were changed and larval body length were decreased in a dose dependent manner of the Pb. The half maximal lethal concentration (LC50) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.58, 0.49, 0.52, 0.54 mg L-1, respectively. The half maximal effective concentration (EC50) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.35, 0.74, 0.30, 0.29 mg L-1, respectively. The teratogenic index (TI) were 1.66 in the Bufo gargarizans, 1.81 in the Hyla japonica, 1.73 in the Rana nigromaculata and 1.86 in the Bombina orientalis, respectively. Therefore, the Pb seems likely to have a teratogenic effect in all four species’ embryonic development. The Bombina orientalis was the most sensitive to the Pb. This means that the difference between the different species, even if they have all been exposed to the same concentration of pollutants depending on the species. The result above show that the Pb acts as a teratogenic agent in the development of the four domestic frog species.
재료 및 방 법
1. 실험동물과 배아 준비
2. 시험물질 처리
3. 결과 분석
결 과
1. 정상발생과정 조사
2. Pb의 농도에 따른 치사율 조사
3. Pb 농도에 따른 기형율 조사
4. Pb 농도에 따른 기형 양상 조사
5. Pb 농도에 따른 성장률 조사
6. Pb에 의한 기형성 지수 조사
고 찰
적 요
REFERENCES
