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pp.1292-1298
The purpose of this study was to evaluate the antioxidant and anti-adipogenic activities of Ligularia stenocephala (L. stenocephala) extract. The contents of the total polyphenol of the extract was 55.950 mg GAE/g residue. Antioxidant activities of L. stenocephala were evaluated by free radical scavenging ability and a reducing power test. 2,2'azino-bis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities of the extract were approximately 90% and 70%, respectively. Reducing power of the extract was 258.833 mg TE/g residue. The anti-adipogenic activity of L. stenocephala extract was examined in 3T3-L1 cells. During adipocyte differentiation, the 3T3-L1 cells were treated both with and without the extract. L. stenocephala extract suppressed the lipid accumulation in a concentration-dependent manner in the 3T3-L1 cells. The L. stenocephala extract inhibited the expression of peroxisome proliferator activated receptor γ (PPARγ) and adipocyte protein 2 (aP2) proteins, compared with control adipocytes. These results indicate that L. stenocephala could be regarded as a potential source natural antioxidant and an anti-obesity agent.
재료 및 방법
1. 재료 및 시약
2. 곤달비 추출물의 제조
3. 총 폴리페놀 함량 측정
4. 라디칼 소거능 측정
5. Reducing power 측정
6. 3T3-L1의 preadipocyte 분화 유도 및 세포독성
7. 세포독성
8. Oil red-O
9. Western blot
10. 통계 처리
결과 및 고찰
1. 곤달비 추출물의 총 폴리페놀 함량
2. 곤달비 추출물의 라디칼 소거능 및 환원력
3. 지방구 형성 측정
4. 지방세포분화 및 전사인자 발현
요약 및 결론
References
