The effect of gamma irradiation on the survival of spore bacteria was investigated in frozen cells (-18℃) with 0.1 M phosphate buffer and inoculated cells in beef. In the case of the frozen cells at log phase, the radiation D_(10) and 12D_(10) values were 0.29 kGy and 3.48 kGy in Bacillus subtilis, 0.39 kGy and 4.68 kGy in Bacillus cereus and 0.46 kGy and 5.52 kGy in Clostridium perfrigens. And inactivation factors were 6.52-10.34 and 10.87-17.24 at the dosage of 3 kGy and 5 kGy, respectively. The radiosensitivity of inoculated cells in beef showed the D_(10) value of 0.59-0.76 kGy, the 12D_(10) value of 7.08-9.12 kGy, and inactivation factors of 3.95-8.47. The radiosensitivity of the frozen cells was higher than that of inoculated cells in beef.

• 김성(한국원자력연구소 방사선식품공학연구실) | Sung Kim
• 육홍선(한국원자력연구소 방사선식품공학연구실) | Hong-Sun Yook
• 최청(영남대학교 식품가공학과) | Cheong Choi
• 김정옥(세종대학교 가정학과) | Jeung-Ok Kim
• 변명우(한국원자력연구소 방사선식품공학연구실) | Myung-Woo Byun