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Transcriptional dynamics of Beauveria bassiana JEF-007 and ERL836 during infection of western flower thrips, Frankliniella occidentalis

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/346920
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

Beauveria bassiana (Bb) is a potential biopesticide against western flower thrips. To understand molecular characteristics of two Bb isolates (ERL836 and JEF-007), whole genome sequencing and RNA-seq were performed. The whole genome of Bb ERL836 was sequenced (3,934,801,443 bp) using Pacbio sequencing and 15 contigs were analyzed. To understand the molecular mechanisms of pathogenicity in two Bb genome (before infection and after infection), next generation sequencing (NGS) was analyzed to compare transcriptomes. A result of differentially expressed gene (DEG) showed that 2,689 contigs were up-regulated and 2,619 contigs were down-regulated. Randomly selected five genes were validated to be highly up-regulated. This work can provide an understanding of the interaction between Bb and westetn flower thrips.

저자
  • Sihyeon Kim(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Korea)
  • Se Jin Lee(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Korea)
  • Mi Rong Lee(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Korea)
  • Jong Cheol Kim(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Korea)
  • So Eun Park(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Korea)
  • Jae Su Kim(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Korea, Plant Medical Research Center, Chonbuk National University, Korea)