Suillus granulatus is an ectomycorrhizal fungus that is associated with pine roots. S. granulatus is edible mushroom and known as the weeping bolete. S. granulatus is similar to S. luteus, but S. granulatus has a ringless stalk. We investigated in the growth characteristics of the 4 strains of S. granulatus with growth medium, temperature, and cellulase enzyme activities. NIFoS 1211 and 2701 showed the highest growth on PDA and NIFoS 1997 produced abundant mycelia on MMN. NIFoS 2615 was grown well on SDA. In spite of same species, the optimal media were different according to habitat of fruit body. The optimal temperature for mycelial growth of all strains was 25oC and NIFoS 2615 was also grown well at 30oC. All of strains on MMN liquid medium were typically grown at pH ranges from 5.8 to 6.2. The dry weights of S. granulatus strains were measured according to different inorganic nitrogen sources. All strains showed the highest dry weight on MMN and showed little difference on none contained nitrogen source, ammonium and nitrate media. We measured cellulase activity on carboxymethylcellulose (CMC) agar plates, and determined clear zone according to pHs. The 4 strains of S. granulatus showed the positive reactions in cellulase activity. All strains formed the height clear zone at pH 6.