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Morphological Characteristics of Protoplast Fusion Strains between Pleurotus abalonus and P. ostreatus
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/348677
The production scale of mushroom cultivation in Korea is approximately 600 billion won, which is 1.6% of the Korean gross agricultural output. Annually, ca. 190,000 tons of mushrooms are harvested in Korea. The oyster mushroom is one of the most favorite and commonly consumed mushrooms in Korea. In case of breeding, the protoplast fusion technique of the oyster mushroom, P. ostreatus was first commercialized in the world. To develop the high temperature varieties, various examinations were accomplished. Protoplast fusion of abalone mushroom, high temperature, and oyster mushroom, popular mushroom, were attempted. 2 strains of P.ostreatus and 2 strains of P.abalonus were fused to each other by protoplast isolation. Fusion strains were investigated by random amplified polymorphic DNA(RAPD) marker, and selected strains were cultivated at 25°C after completing the mycelial growth. As a result of sawdust bag cultivation, most of strains showed the fruiting body, but the morphological characteristics among them were not significant different. However, these protoplast fusion strains were expected as new parents strains to develop varieties.
