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Isolation and Characterization of GSTs Gene Homology Sequences from Agropyron mongolicum Keng

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  • URLhttps://db.koreascholar.com/Article/Detail/349670
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한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
초록

In this study, GSTs gene homology fragment from Mongolian wheatgrass(Agropyron mongolicum Keng) was isolated using homology-based method. one partials gene sequences have been obtained by reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis using Software of DNAman and DNAuser etc, and showed that the cDNA sequences was 344 base pairs, encoding 62 amino acids. The partial gene had C-terminal conserved domains of substrate binding pocket (H-site) of GSTs superfamily. Homology comparison with GSTs gene amino acid sequences in other plants showed that it was 91% identical to 19E50 of wheat, 90% to pk0078 and 88% to gstf6. It was named as MwGSTs.

저자
  • Yan Zhao
  • Jin-feng Yun
  • De-Fu Liu