P. linteus(품종명: 고려상황, PLKS)와 P. baumii (장수 상황, PBJS)를 동일한 조건하에서 인공재배하여 얻어진 자실체를 70% 메탄올, 60% 에탄올, 열수추출하여 폴리페놀과 플라보노이드 측정 시 함량은 60%에탄올 추출물에서 가장 높게 나타났다. 60%에탄올을 용매로 PLKS와 PBJS의 자실체를 추출하여 농도별로 DPPH (2,2-diphenyl- 1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbisthiazoline- 6-sulfonic acid) 라디칼 소거능을 측정한 결과 PLKS와 PBJS는 거의 유사한 항산화 활성을 보였으며 자실체가 균사체보다 2-3배 이상 높은 항산화 활성이 측정되었으나 FRAP (ferric reducing antioxidant power)분석에서는 자실체와 균사체 간에 큰 차이가 없는 것을 확인하였다. PLKS와 PBJS의 자실체 ethyl acetate 분획물을 HPLC 분석한 결과 styrylpyrone 계 폴리페놀 성분인 davallialactone, hispidin, hypholomine B, inoscavin A를 검출할 수 있었으나 균사체에서는 검출되지 않아 자실체 특이적인 화합물인 것을 확인하였다.

P. linteus (Korea Sanghwang, PLKS) and P. baumii (Jangsoo Sangwhang, PBJS) were artificially cultivated under the same conditions. Fruiting bodies were extracted using 70% methanol, 60% ethanol, and hot water. Phenol and flavonoid contents were optimally extracted using 60% ethanol. Antioxidant activities of 60% ethanol extracts from fruiting bodies and mycelia from each Sanghwang mushroom species were measured using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3- ethylbisthiazoline-6-sulfonic acid) radical scavenging activities, and FRAP (ferric reducing antioxidant power). The antioxidant activities of fruiting bodies were relatively higher in comparison to those of mycelial samples. In high performance liquid chromatography (HPLC) analysis, styrylpyrone-class poly phenolic compounds, davallialactone, hispidin, hypholomine B, and inoscavin A were detected in fruiting body samples of two Sanghwang mushroom species, but not in their mycelial samples.

ABSTRACT
서 론
재료 및 방법
    공시 균주 및 배양
    원목재배 및 자실체 생산
    상황버섯 자실체 추출물
    총 폴리페놀 함량 측정
    총 플라보노이드 함량 측정
    DPPH 라디칼 소거능 활성 측정
    ABTS 라디칼 소거능 활성 측정
    FRAP를 이용한 총 항산화력 측정
    통계처리
    HPLC 분석
결과 및 고찰
    진흙버섯 종의 형태적 특성
    상황버섯 종의 총 폴리페놀과 플라보노이드 함량
    상황버섯 종의 항산화 활성
    폴리페놀 성분의 HPLC 분석
적 요
REFERENCES

• 민경진(농촌진흥청 인삼특작부 버섯과) | Gyeong-jin Min (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
• 윤봉식(전북대학교 생명공학부) | Bong-Sik Yun (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
• 강희완(한경대학교 원예생명공학과/한경대학교유전공학연구소) | Hee-Wan Kang (Department of Horticultural Biotechnology, Hankyong National University/Institute of Genetic Engineering, Hankyong National University) Corresponding author