Glyphosate, a worldwide most used herbicides, can have complex implications for terrestrial ecosystems, extending beyond its intended target, weed control. This study investigated the cascading effects of glyphosate application on both plant and collembolan communities. Our findings reveal that glyphosate application can reduce the richness of plant communities, and these effects can be pronounced in areas with low initial plant diversity. Furthermore, our results confirm that the fluctuations in plant community composition induced by glyphosate application can also impact the richness of collembolan communities. This research highlights the importance of considering both plant and invertebrate communities when assessing the impacts of herbicide use in agroecosystems.

• June Wee(OJeong Resilience Institute, Korea University, Seoul 02841, Republic of Korea) Corresponding author
• Yun-Sik Lee(Department of Biology Education, Pusan National University, Busan 46241, Republic of Korea)
• Yongeun Kim(OJeong Resilience Institute, Korea University, Seoul 02841, Republic of Korea)
• Yong Ho Lee(OJeong Resilience Institute, Korea University, Seoul 02841, Republic of Korea)
• Sunhee Hong(Department of Plant Resources and Landscape, Hankyong National University, Anseong 17579, Republic of Korea)
• Kijong Cho(OJeong Resilience Institute, Korea University, Seoul 02841, Republic of Korea, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea)