Using the freezing removal method, we investigated residual pesticides in 50 grape seed oils distributed in Gyeonggi Province, South Korea. The fat was mixed with acetonitrile and then frozen at ≤-20oC for 24 h. Fats and oils were removed by separating those in solid state and the extract acetonitrile in liquid state. Ten residual pesticides were detected 161 times in 49 of 50 cases. The detected pesticides were boscalid, cyclufenamide, deltamethrin, difenoconazole, fluxapyroxad, fenpyrazamine, kresoxim-methyl, piperonyl butoxide, tebuconazole, and trifluoxysorbin. Boscalid, a fungicide, was most frequently detected (44 times), followed by fluxapiroxad (35 times). The detection range was 0.01-1.10 mg/kg, which was within the legal limit of residual pesticide for grapes. The recovery rate of the detected pesticides was 72.6-129.8% and the ratio of estimated daily intake/acceptable daily intake was calculated to determine the risk of the detected pesticides, which was <0.0028%. This indicated that the risk caused by pesticide residues in grape seed oil is at a safe level.

경기도에서 유통중인 포도씨유 50건을 대상으로 잔류농 약 실태를 조사하였다. 50건 중 49건에서 10종의 잔류농 약이 161회 검출되었다. 검출된 농약은 boscalid, cyflufenamid, deltamethrin, difenoconazole, fluxapyroxad, fenpyrazamine, kresoxim-methyl, piperonyl butoxide, tebuconazole, trifloxystrobin 으로 살충제 2종, 살균제 8종이었으며 살균제인 boscalid가 44회, fluxapyroxad가 35회로 가장 빈번하게 검출되었다. 검 출범위는 0.01-1.10 mg/kg으로 모두 포도의 잔류농약 허용 기준 이내로 검출되었다. 검출된 농약의 회수율은 72.6- 129.8%이었으며 검출된 농약의 위해성을 알아보기 위해 %ADI를 산출한 결과 0.0028% 이하로 나타나 포도씨유의 잔류농약으로 인한 위해성은 안전한 수준으로 조사되었다. 그러나 본 연구는 시료의 수가 적고 GC/MS/MS로 분석가 능한 농약만 조사하였기 때문에 보다 신뢰성 있는 결과를 얻기 위해선 앞으로도 꾸준한 추적 조사가 필요할 것으로 사료된다.

Materials and methods
    시약 및 재료
    분석기기 및 조건
    분석방법
    유효성 검증
    노출량 조사
Results and discussion
    유효성 검증
    포도씨유의 잔류농약 조사 결과
    검출 농약의 노출량 조사
국문요약
References

• 손미희(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | Mi-Hui Son (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea) Corresponding author
• 김재관(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | Jae-Kwan Kim (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea)
• 이유진(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | You-Jin Lee (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea)
• 김지은(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | Ji-Eun Kim (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea)
• 백은진(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | Eun-Jin Baek (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea)
• 김병태(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | Byeong-Tae Kim (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea)
• 박명기(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | Myoung-Ki Park (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea)
• 권보연(경기도보건환경연구원 농수산물검사부 안산농수산물검사소) | Bo-yeon Kwon (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment, Ansan, Korea)