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체외수정 및 미세조작에 의한 가축(胚)의 생산과 효율적 이용에 관한 연구 III. 소에 있어서 난포란의 체외수정과 수정란 이식 KCI 등재
Studies on Production and Efficient Utilization of Livestock Embryos by In Vitro Fertilization and Micromanipulation. III. Transfer of Embryo Derived from In Vitro Fertilization of Bovine Follicular Oocytes Matured In Vitro
pp.261-268
Immatured bovine follicular oocytes added with serum, hormones, granulosa cells and bovine oviduct epithelium cells were fertilized in vitro after in vitro maturation. In vitro maturation and early development capacity were examined and IVF-derived embryos were transferred and to recipients and effects of sperm treatment on in vitro capacitation were investigated. The rate of in vitro maturation was improved when they were co-culutred with granulosa cells in the TCM199 medium added with 10% FCS and hormones. The percentage of acrosome reaction was not differed between sperm treatments and sperm of above 25% under-went AR during 30 min preincubation with caffeine and heparin. The cleavage rate of oocytes in vitro fertilized in TCM199 medium added with 10% FCS and hormones, GC or BOEG higher than that in medium with 10% FCS and GC. But the rate was not significantly different between GC and BOEG The cleavage of rate oocytes cultured in medium containing serum, hormones and BOEG was 80.2% and more embryos were developed to Blastocyst (17.3%). The selected embryos were transferred to 9 recipients by surgical or nonsurgical method but did not result in pregnancy.
