논문 상세보기
pp.145-152
유전적으로 우수한 능력을 보유하고 있으나 정상적으로 번식이 불가능한 젖소 16두를 간이 난자 체취기를 이용하거나 장기공태 젖소 5두를 초음파 난자체취기를 이용하여 미성숙난자를 채취하므로서 체외수정란을 안정적으로 생산하기 위해 채취기기의 효율성과 반복채란에 미치는 영향을 조사한 결과는 다음과 같다. 1 공란우당 채취회수는 간이 난자 체취기 8.8회였고, 초음파기기는 8.8회였으며, 공란우당 평균 회수된 난포란은 각각 4.39개와 4.14개로 채취기기간에
Transvaginal ovum pick-up (OPU) offers several advantages to standard embryo transfer procedures. For a systematic comparison of the ultrasound-guided (UG) and the hand-operated vacuum pump (HP) OPU method, the repeated session and mean number of heads between the UG and HP methods were 8.8 and 8.3, and 5 and 16, respectively. The efficacy of oocyte retrieval was same to long time OPU by UG vs HP for retrieval oocytes in Holstein cows. The number of retrieval oocytes was 4.143.25 (UG) and 4.393.61 (HP). No significant differences (P<0.05) for the retrieval oocyte numbers were observed in the methods. The oocytes rates thor the grade I or II oocyte for HP and UG were 51.3% and 59.9%, respectively. There was no significant difference (P<0.05) in oocyte collection rate for the grade of I or II. In the long term OPU. important variation was found to ovary adhesion that was observed in two heads in the HP. In concussion. the use of UC or HP machine for long term oocyte retrieval may be possible for corms which have inability for reproduction.
