논문 상세보기
pp.25-33
본 연구는 가축유전자원의 효율적 보존방법의 개발을 위해 수행되는 체외 정자 세포 생산 연구의 일부로, 생산된 정자 세포의 발생능을 검사하기 위한 체외배양 시스템을 확립 할 목적으로 수행되었다. 성숙 배양시간을 48시간으로 하여 ethanol로 활성화처리한 후 TCM199에 의 소 태아 혈청으로 배양하였을 때 배반포까지 발달하지 못하였으나, NCSU23에 소 혈청 알부민으로 배양하였을 때 의 활성화된 난모세포가 배반포기까지 발달하였다. 성숙시간을 연장하여
This study is a part of research that development of effective genetic resources preservation system using the in vitro spermatogenesis, in vitro insemination and culture system. We aimed for establishment of in vitro culture system with in vitro activated porcine oocytes. The porcine oocytes were matured for 48 hours in FCS and activated with ethanol. The activated oocytes were cultured for 7 days in FCS or BSA medium. The activated oocytes were not developed to the blastocyst stage in FCS medium. However in medium, those were developed to blastocyst with of treated oocytes. We extended maturation duration of porcine follicular oocytes fur 48, 52, 56, 60, 64, 68, and 72 hours and activated with ethanol and cultured using BSA medium. The six percents of activated oocytes were developed to blastocyst in 48 hours and in 52 hours with comparatively low rates suggested to be not fully activated by regenerated MPF. Maturation durations from 56 hours to 68 hours supported to develop upto of blastocysts. However the developmental rate was declined to at 72 hours of maturation duration because of cytoplasmic deterioration. The assumed time window for activation will be hours of maturation duration. When the matured oocytes were activated with electric pulse of 1, 1.2, 1.4, 1.6, 1.8 and 2.0kV/cm for , although appling the electric current once was not enough for activation, appling twice with 1.6kV/cm for was shown the highest developmental rate with . When those were compared with activating methods, of blastocyst rate was obtained in the ethanol. That was higher than those in electric pulse with and calcium ionophore method with . In this experimental condition, the ethanol treatment was the most effective method for activating porcine oocytes.
