한국응용곤충학회 학술대회논문집 2008년도 정기총회 및 춘계학술발표회 (p.10-10)

|소모임(산업곤충연구회, 곤충분류연구회)|
Bombyx mori Protein Disulfide Isomerase Enhances the Production of Nuecin, an Antibacterial Protein

키워드 :
Baculovirus expression vector system,BEVS,Bombyx mori,Nuecin,Protein,disulfide isomerase,PDI

초록

The insect baculovirus expression vector system (BEVS) is useful for the production of biologically active recombinant proteins. However, the overexpression of foreign proteins in this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we have developed a versatile baculovirus expression and secretion system using the Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion improved the secretion and antibacterial activity of recombinant nuecin proteins. Thus, bPDI gene fusion is a useful addition to the BEVS for the large-scale production of bioactive recombinant proteins.