The insect baculovirus expression vector system (BEVS) is useful for the production of biologically active recombinant proteins. However, the overexpression of foreign proteins in this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we have developed a versatile baculovirus expression and secretion system using the Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion improved the secretion and antibacterial activity of recombinant nuecin proteins. Thus, bPDI gene fusion is a useful addition to the BEVS for the large-scale production of bioactive recombinant proteins.

• Tae Won Goo(Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
• Eun Young Yun(Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
• Sung Wan Kim(Department of Anatomy, College of Medicine, Chungnam National University)
• Kwang Ho Choi(Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
• Min Uk Kang(Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
• O-Yu Kwon(Department of Anatomy, College of Medicine, Chungnam National University)
• Seok Woo Kang(Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)