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고순도 Galactooligosaccharide 제조 및 유산균 증식 활성 KCI 등재

Preparation of High Purity Galacto-Oligosaccharide and Its Prebiotic Activity In Vitro Evaluation

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

This study attempted to find an efficient method for the preparation of high-purity galactooligosaccharides (HP-GOS) using β-galactosidase and yeast fermentation. GOS prepared using Lactozym 3000L showed the greatest enhancement in total GOS of the six β-galatosidases tested. GOS alone achieved 51% conversion of initial lactose. GOS production was enhanced by fermentation with commercial yeast (Saccharomyces cerevisiae); its concentration reached 71% after 36h fermentation with 8% yeast. Component sugar analysis with HPLC indicated that HP-GOS fermented with S. cerevisiae showed significantly increased levels of 4’/6’-galactosyllactose and total GOS as well as a significantly decreased glucose level. HP-GOS facilitated the growth of Lactobacillus sp. (L. acidophilus and L. casei) and Bifidobacterium sp. (B. longum and B. bifidum). In sum, high-purity GOS has been successfully produced through both an enzymatic process and yeast fermentation. GOS encourages the growth of bacteria such as Lactobacillus and Bifidobacterium that may be beneficial to human gastrointestinal health.

저자
  • 홍기배(고려대학교 대학원 보건과학과) | Ki Bae Hong
  • 서형주(고려대학교 대학원 보건과학과) | Hyung Joo Suh
  • 김재환(네오크레마) | Jae Hwan Kim
  • 권혁권(네오크레마) | Hyuk Kon Kwon
  • 박청(대전대학교 링크사업단) | Chung Park
  • 한성희(고려대학교 생물신소재연구소) | Sung Hee Han Corresponding author