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Zanthoxylum piperitum DC 당단백질의 첨가에 따른 Lactobacillus plantarum L67 유도단백질의 생리활성에 미치는 영향 KCI 등재후보

Antioxidant Activity of Induced Protein of Lactobacillus plantarum L67 Supplemented with Zanthoxylum piperitum DC Glycoproteins

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Current Topic in Lactic Acid Bacteria and Probiotics (한국유산균·프로바이오틱스학회지)
한국유산균·프로바이오틱스학회 (Korean Society for Lactic Acid Bacteria and Probiotics)
초록

This study was to investigate whether the bioactivity of L. plantarum L67 can be enhanced by treatment with Zanthoxylum piperitum DC (ZPDC) glycoprotein or compounds of similar molecular weights (24 kDa). When L. plantarum L67 was treated with ZPDC glycoprotein of various concentrations, the intensities of a few SDS-PAGE bands were slightly changed. The amount of a 23-kDa protein was increased upon treatment with increasing concentrations of ZPDC glycoprotein. The activities of antioxidant enzymes (SOD, GPx, and CAT), growth rate, and β-galactosidase activity were more increased at treatment with ZPDC glycoprotein than at treatment of L. plantarum L67 only, whereas the ATPase activity was decreased. Interestingly, it did not affect scavenging activity for the DPPH. The founding result suggest that, one of beneficial strategies for increase the bioactivity of L. plantarum L67 might be to take ZPDC.

목차
서 론
 재료 및 방법
  ZPDC 당단백질의 제조
  L. plantarum L67의 배양
  L. plantarum L67 단백질의 추출
  iL67 단백질의 세포 독성
  효소 활성 평가
  ATPase 활성 평가
  β-galactosidase 활성 평가
  통계 분석
 결과 및 고찰
  ZPDC 처리로의 L. plantarum L67의 유도 효과
  Induced L. plantarum 67 (iL67) 단백질의 항산화 활성
  L. plantarum L67의 ZPDC 처리에 의한 Raw 264.7 세포 내에서 항산화 효소의 조절
  ZPDC 당단백질의 L. plantarum L67의 성장에 대한 효과
  ZPDC 당단백질 처리에 의한 L. plantarum L67의 ATPase활성 변화
  ZPDC 당단백질 처리에 의한 L. plantarum L67의 β-galactosidase 활성 변화
 요 약
 감사의 글
 참고문헌