The vitamin E enhanced transgenic soybean was developed by introducing a perilla γ- tocopherol methyltransferase gene (γ-TMT) under the control of pea vicilin promoter and a selection marker, phosphinothricin acetyltransferase (PAT) gene. With regard to the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. We studied the effects of the vitamin E enhanced transgenic soybean feeding on survival of Daphnia magna which is commonly used as a model organism in ecotoxicological studies. The Daphnia magna was fed on vitamin E enhanced transgenic soybean and non-genetically modified (non-GM) soybean (Willams 82) at 0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L concentrations, respectively. The GM soybean used for the test was confirmed to have the γ-TMT/PAT gene expression by the enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) analysis. The results showed that there was no significant differences between cumulative immobilities of Daphnia magna fed on GM soybean and non-GM soybean. The 48hr-EC50 values showed no significant differences between GM soybean (2,416 mg/L) and non-GM soybean (2,408 mg/L). The results of this study suggested that there was no significant differences in toxicity for Daphnia magna between GM soybean and non-GM counterpart.

재료 및 방법
  시료 제조
  Genomic DNA 추출 및 도입유전자 Polymerase chainreaction (PCR) 분석.
  환경 방출 조건에 따른 비타민 E 강화콩의 목적 단백질함량 분석
  비타민 E 강화콩의 PAT 단백질 분석
  급성독성 평가를 위한 물벼룩 배양 조건
  물벼룩 급성독성 시험의 비타민 E 강화콩과 Willams 82처리 조건
  물벼룩 급성독성 평가를 위한 조사항목
 결과 및 고찰
  비타민 E 강화콩의 분자생물학적 분석
  비타민 E 강화콩의 노출 조건과 시기별 목적 단백질 함량분석
  물벼룩 급성독성 시험 용수의 수질 분석
  비타민 E 강화콩과 Willams 82의 처리농도에 따른 물벼룩의 유영 저해 분석
  비타민 E 강화콩과 Willams 82의 급이에 의한 물벼룩의급성 독성
 적 요
 REFERENCES

• 오성덕(농촌진흥청 국립농업과학원 생명자원부) | Sung-Dug Oh
• 윤도원(농촌진흥청 국립농업과학원 생명자원부) | Doh-Won Yun
• 손수인(농촌진흥청 국립농업과학원 생명자원부) | Soo-In Sohn
• 이범규(농촌진흥청 국립농업과학원 생명자원부) | Bum Kyu Lee
• 이기종(농촌진흥청 연구운영과) | Kijong Lee
• 장안철(농촌진흥청 국립농업과학원 생명자원부) | AnCheol Chang