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병원성 미생물에 대한 Lactobacillus bulgaricus SP5의 항균활성 KCI 등재

Antibacterial Activity by Lactobacillus bulgaricus SP5 against Pathogenic Bacteria

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한국유기농업학회지 (Korea Journal of Organic Agriculture)
한국유기농업학회 (Korea Association Of Organic Agriculture)
초록

This study was carried out to get basic resources for the industrial use of Lactobacillus bulgaricus SP5. The antibacterial activity of the supernatant obtained from Lactobacillus bulgaricus SP5 was tested against the pathogenic bacteria such as Escherichia coli KCCM 11234, Salmonella enteritidis KCCM 3313, Salmonella enteritidis KCCM 12021, and Salmonella typhimurium KCCM 40253. The supernatant of L. bulgaricus SP5 showed antibacterial activity against tested pathogenic bacteria. The antibacterial activity was examined after adjusting pH and heat treatment of supernatant. Heat treatment of supernatant had antibacterial activity against pathogenic bacteria at all temperature. However, pH changes showed no antibacterial activity. Antibacterial activity of the supernatant was confirmed to be due to organic acids (lactic, acetic, phosphoric, succinic, pyroglutamic, citric, malic, and formic acid).

목차
Ⅰ. 서 론
 Ⅱ. 재료 및 방법
  1. 균주
  2. 시약 및 재료
  3. L. bulgaricus SP5의 배양
  4. 항균활성 측정
  5. 전기영동
  6. Tricine-SDS-PAGE에 의한 항균물질 검출
  7. pH 안정성
  8. 열 안정성
  9. HPLC를 이용한 유기산분석
  10. 통계처리
 Ⅲ. 결과 및 고찰
  1. Lactobacillus bulgaricus가 분비하는 항균물질
  2. 전기영동과 Tricine-SDS-PAGE에 의한 항균물질 검출
  3. pH 안정성
  4. 열 안정성
  5. HPLC를 이용한 유기산 분석
 Ⅳ. 적 요
 References
저자
  • 김완섭(한경대학교 동물생명환경과학과) | Kim Woan-Sub Corresponding author
  • 양아름(한경대학교 동물생명환경과학과) | Yang A-Reum