Barley yellow mosaic virus (BaYMV) is transmitted by a root-inhabiting Polymyxa graminis (P. graminis) and thus the disease is called “soil-borne”. In this study, the presence of P. graminis was confirmed by PCR test based on specific sequences of P. graminis type Ⅰ (P. graminis f. sp. temperata) Internal Transcribed Spacer (ITS). P. graminis was detected in the BaYMV infected soil and root of barley plants. The monitoring of P. graminis was conducted in March 2015 in 8 korean provinces. It was detected in the soil of all collected regions. This is the first report on a P. graminis a survey of Korea.

Ⅰ. 서 론
 Ⅱ. 재료 및 방법
  1. 매개곰팡이(P. graminis) 진단 특이 primer 제작
  2. 지역별 토양시료 채집
  3. 이병 토양으로부터 P. graminis DNA 분리 및 PCR
  4. BaYMV RT-PCR 검정
 Ⅲ. 결과 및 고찰
  1. 매개곰팡이 검정방법 확립
  2. 2015년도 보리 바이러스병 분포 현황 및 지역별 P. graminis의 분포
 Ⅳ. 요 약
 References

• 이봉춘(농촌진흥청 국립식량과학원) | Lee Bong-Choon Corresponding author
• 김상민(농촌진흥청, 국립식량과학원) | Kim Sang-Min
• 배주영(농촌진흥청, 국립식량과학원) | Bae Ju-Young
• 나지은(농촌진흥청, 국립식량과학원) | Ra Ji-Eun
• 김선림(농촌진흥청, 국립식량과학원) | Kim Sun-Lim
• 김강민(전북대학교 생명공학부) | Kim Kang-Min
• 이중환(경상북도 농업기술원 생물자원연구소) | Lee Joon-Hwan