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UHPLC를 이용한 볏짚 사일리지와 동계사료작물의 오크라톡신과 제랄레논 분석법 최적화 KCI 등재

Optimization of Analytical Methods for Ochratoxin A and Zearalenone by UHPLC in Rice Straw Silage and Winter Forage Crops

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한국초지조사료학회지 (Journal of The Korean Society of Grassland Science)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
초록

The objective of this study was to optimize analytical methods for ochratoxin A (OTA) and zearalenone (ZEA) in rice straw silage and winter forage crops using ultra-high performance liquid chromatography (UHPLC). Samples free of mycotoxins were spiked with 50 μg/kg, 250 μg/kg, or 500 μg/kg of OTA and 300 μg/kg, 1500 μg/kg, or 3000 μg/kg of ZEA. OTA and ZEA were extracted by acetonitrile and cleaned-up using an immunoaffinity column. They were then subjected to analysis with UHPLC equipped with a fluorescence detector. The correlation coefficients of calibration curves showed high linearity (R2 ≧ 0.9999 for OTA and R2≧0.9995 for ZEA). The limit of detection and quantification were 0.1 μg/kg and 0.3 μg/kg, respectively, for OTA and 5 μg/kg and 16.7 μg/kg, respectively, for ZEA. The recovery and relative standard deviation (RSD) of OTA were as follows: rice straw = 84.23~95.33%, 2.59~4.77%; Italian ryegrass = 79.02~95%, 0.86~5.83%; barley = 74.93~97%, 0.85~9.19%; rye = 77.99~ 96.67%, 0.33~6.26%. The recovery and RSD of ZEA were: rice straw = 109.6~114.22%, 0.67~7.15%; Italian ryegrass = 98.01~109.44%, 1.65~4.81%; barley = 98~113.53%, 0.25~5.85%; rye = 90.44~108.56%, 2.5~4.66%. They both satisfied the standards of European Commission criteria (EC 401-2006) for quantitative analysis. These results showed that the optimized methods could be used for mycotoxin analysis of forages.

저자
  • 함현희(국립농업과학원 유해생물팀) | Hyeonheui Ham
  • 문혜연(국립농업과학원 유해생물팀) | Hye Yeon Mun
  • 이경아(국립농업과학원 유해생물팀) | Kyung Ah Lee
  • 이수형(국립농업과학원 유해생물팀) | Soohyung Lee
  • 홍성기(국립농업과학원 유해생물팀) | Sung Kee Hong
  • 이데레사(국립농업과학원 유해생물팀) | Theresa Lee
  • 류재기(국립농업과학원 유해생물팀) | Jae-Gee Ryu Corresponding author