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에티디움브로마이드와 자외선 처리로 제조한 붉은자루동충하초 돌연변이체의 세포외 단백질분해효소 생산 및 곤충 큐티클 분해활성 증진

Generation of Cordyceps pruinosa Mutants by Combined Treatment of Ethidium Bromide and Ultra-Violet (UV), Allowing Enhanced Extracellular Acid Protease Production and Inscet Cuticle-Degrading Activity

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  • URLhttps://db.koreascholar.com/Article/Detail/326431
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : The purpose of the present investigation is to enhance extracellular acidic protease production by subjecting a protease producing strain Cordyceps pruinosa DK-01 to random mutagenesis by UV irradiation after ethidium bromide treatment. Methods and Results : Mutants were screened as protease producers on the basis of zone of clearance and relative proteolytic activity (RPA) on skimmed milk agar plates. In addition, mutants showed strong pink-red color intensity and different RAPD profiling compared with wild type control. Four mutants were randomly selected and their extracellular enzyme activities were investigated. In liquid culture without casein, 2.2-, 2.9-, 5.2- and 4.4-fold higher acid protease activity was achieved from mutants DK-m9, -m11 and -m12, respectively, than that of wild type strain (11.13 ± 1.60 U/ml). In liquid culture with casein, 1.1-, 1.3-, 1.3 and 1.3-fold higher acid protease activity was achieved with those mutants were found to produce, respectively, than that of wild type strain (93.95 ± 12.84 U/ml). Maximum acid protease activity was noticed from a mutant DK-m11 in liquid culture with casein (121.18 U/ml) and without casein (57.65 U/ml). The extracellular acid protease produced from DJ-m11 that was active in the pH range 4.5-6.5 and optimum temperature for the activity was 37°C. Furthermore, we found a deformed, shorten structure of setae on the elytron surface of dynastid beetles treated with culture supernatant of the DK-m11. Conclusion : These findings have more impact on enzyme economy for biotechnological and insecticidal applications of fungal proteases.

저자
  • Hyun Jung Park(Department of Biology and Medicinal Science, Pai Chai University) | 박현정
  • Sung Oh(Department of Biology and Medicinal Science, Pai Chai University) | 오성
  • Jung Mo Koo(Department of Biology and Medicinal Science, Pai Chai University) | 구정모
  • Seong Mi Ji(Department of Biology and Medicinal Science, Pai Chai University) | 지성미
  • Han Byul Noh(Department of Biology and Medicinal Science, Pai Chai University) | 노한별
  • Chang Won Choi(Department of Biology and Medicinal Science, Pai Chai University) | 최창원 Corresponding author