논문 상세보기

간세포 독성과 에탄올 대사에서 추출 조건에 따른 다슬기 추출물의 효과 KCI 등재

Effect of Semisulcospira libertina Extracts from Different Extraction Processes on Liver Cell Toxicity and Ethanol Metabolism

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/326806
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
산업식품공학 (Food Engineering Progress)
한국산업식품공학회 (Korean Society for Food Engineering)
초록

Although Semisulcospira libertina is generally regarded as a supplement for the alleviation of alcohol hangover, little is known about its effects on cell metabolism. Therefore, this study was conducted to analyze the constituents of the extracts prepared using different extraction methods and to compare their biochemical properties. The amino acid contents were found to be much higher in acidic and enzymatic hydrolysates than hot water extracts from S. libertina. DPPH radical scavenging activities in acidic and enzymatic hydrolysates were higher than those of hot water extracts. Three types of S. libertina hydrolysate was added to HepG2 cells damaged by acetaminophen (AAP), after which the survival rate of HepG2 cell were measured. In addition, lactate dehydrogenase (LDH) activities in the culture media were evaluated. The survival rates of HepG2 cells were 77.0±4.3% and 81.5±1.3% at 3 h and 5h enzymatic hydrolysates, respectively. These cell survival rates were higher compared to those of the negative control group (67.8±4.3%) treated only with acetaminophen. Cellular toxicities induced by treatment with AAP were also significantly alleviated in response to treatment with the extracts of S. libertina. In addition, the activities of 2 key enzymes that metabolize ethanol, alcohol dehydrogenase and aldehyde dehydrogenase, were upregulated by 4.7- and 2.7-fold respectively in response to treatment with a 3 h enzymatic hydrolysate of S. libertina. Taken together, these results provide biochemical evidence of the method by which S. libertina exerts its biological functions, including the alleviation of alcohol hangover and the protection of liver cells against toxic insults.

저자
  • 조경환((재)하농녹차연구소)
  • 추호진((주)정옥)
  • 서민균((주)정옥)
  • 김종철((재)하농녹차연구소)
  • 신유진((재)하농녹차연구소)
  • 류기형(공주대학교 식품공학과)
  • 조희영(경상대학교 의과대학 외과학교실)
  • 정치영(경상대학교 의과대학 외과학교실)
  • 하영술(경상대학교 의과대학 외과학교실) Corresponding author