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Enhanced Susceptibility to Brucella abortus Infection in Dextran Sodium Sulfate Treated Human Cervical Cancer HeLa Cells KCI 등재

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농업생명과학연구 (Journal of Agriculture & Life Science)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
초록

Treatment of dextran sodium sulfate(DSS) on HeLa cells results to an enhanced susceptibility to Brucella(B.) abortus infection. An increase in the adherence, invasion and intracellular replication of B. abortus was observed in DSS-treated cells. Furthermore, a marked elevation in the intensity of F-actin fluorescence was also observed in DSS-treated cells compared with untreated B. abortus-infected cells. An upregulation of phagocytic signaling proteins by Western blot analysis demonstrated an apparent activation of ERK, p38α and JNK phosphorylation levels in B. abortus-infected DSS-treated cells compared with the control. Colocalization with LAMP-1 proteins was attenuated in DSS-treated cells upon intracellular trafficking of the pathogen compared with control cells. The results of this study demonstrated consistency with other pathogens. The uptake and intracellular replication of B. abortus is hypothesized to be stimulated by various dextran receptors such as C-type lectins that are involved in phagocytosis which can either be direct phagocytic receptors, modulators of the expression of other receptors or as opsonins leading to an enhanced internalization of B. abortus. The complexity of these interactions thus would warrant further investigation into the role of DSS in the pathogenesis of brucellosis. In summary, we conclude that DSS enhanced adhesion, phagocytosis and intracellular replication of B. abortus in epithelial cells which could lead to suppression of the innate immune system in chronic Brucella infection.

저자
  • Lauren Togonon Arayan(nstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
  • Alisha Wehdnesday Bernardo Reyes(nstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
  • Huynh Tan Hop(nstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
  • Huy Tran Xuan Ngoc(nstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
  • Suk Kim(Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 52828, Korea) Corresponding author