DNA-based markers have been used in various fields like molecular biology and crop breeding program. DNA marker is able to be used for protection of genetic resource and quantification of specific cultivar. Eleven DNA markers have been developed for Korean wheat cultivar identification in 2013-2014. 27 of 32 wheat cultivars were distinguished by 11 DNA markers. In this study, we developed four DNA markers, KWSM0012, KWSM0013, KWSM0014 and KWSM0015, derived from SSR and SNP analysis. Consequently, 32 Korean wheat cultivars were identified by 15 DNA markers. We are convinced that these new DNA markers are very useful for wheat varieties DNA fingerprinting and are able to be applied to marker-assisted selection in wheat breeding program in Korea.

• Jae-Han Son(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea)
• Young-Keun Cheong(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea)
• Jong-Chul Park(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea)
• Yang-Kil Kim(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea)
• Kyong-Ho Kim(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea)
• Tae-Il Park(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea)
• Bo-kyeong Kim(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea)
• Chon-Sik Kang(Crop Breeding Division, National Institute of Crop Science, RDA, Wanju, 55365, Korea) Corresponding author